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Promega pgemt-easy sequencing vectors
Pgemt Easy Sequencing Vectors, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgemt-easy+sequencing+vectors/pgem+t+easy/pmc10373592-99-13-16
Average 90 stars, based on 1 article reviews
pgemt-easy sequencing vectors - by Bioz Stars, 2026-10
90/100 stars

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Polymerase Chain Reaction:

Article Title: Multi-allelic gene editing in an apomictic, tetraploid turf and forage grass ( Paspalum notatum Flüggé) using CRISPR/Cas9
Article Snippet: The 586 bp PCR products were purified using GeneJet PCR Purification Kit (ThermoFisher; MA, USA), and 200 ng of each purified product was incubated with 0.5 μL of Pvu II-HF (New England Biolabs) or Kfl I (ThermoFisher) for 16 h at 37°C and electrophoresed as described. .. PCR products from putatively edited lines were subjected to A-tailing and cloned into pGEMT-Easy sequencing vectors (Promega; WI, USA) according to the manufacturer’s protocols. .. Cloned PCR amplicons of the targeted MgCh gene were amplified in 10-beta chemically competent E. coli cells (New England Biolabs) and purified with a GeneJet Plasmid Miniprep Kit (ThermoFisher).

Clone Assay:

Article Title: Multi-allelic gene editing in an apomictic, tetraploid turf and forage grass ( Paspalum notatum Flüggé) using CRISPR/Cas9
Article Snippet: The 586 bp PCR products were purified using GeneJet PCR Purification Kit (ThermoFisher; MA, USA), and 200 ng of each purified product was incubated with 0.5 μL of Pvu II-HF (New England Biolabs) or Kfl I (ThermoFisher) for 16 h at 37°C and electrophoresed as described. .. PCR products from putatively edited lines were subjected to A-tailing and cloned into pGEMT-Easy sequencing vectors (Promega; WI, USA) according to the manufacturer’s protocols. .. Cloned PCR amplicons of the targeted MgCh gene were amplified in 10-beta chemically competent E. coli cells (New England Biolabs) and purified with a GeneJet Plasmid Miniprep Kit (ThermoFisher).

Sequencing:

Article Title: Multi-allelic gene editing in an apomictic, tetraploid turf and forage grass ( Paspalum notatum Flüggé) using CRISPR/Cas9
Article Snippet: The 586 bp PCR products were purified using GeneJet PCR Purification Kit (ThermoFisher; MA, USA), and 200 ng of each purified product was incubated with 0.5 μL of Pvu II-HF (New England Biolabs) or Kfl I (ThermoFisher) for 16 h at 37°C and electrophoresed as described. .. PCR products from putatively edited lines were subjected to A-tailing and cloned into pGEMT-Easy sequencing vectors (Promega; WI, USA) according to the manufacturer’s protocols. .. Cloned PCR amplicons of the targeted MgCh gene were amplified in 10-beta chemically competent E. coli cells (New England Biolabs) and purified with a GeneJet Plasmid Miniprep Kit (ThermoFisher).



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